Cryoelectron Microscopy of Macromolecular Assemblies

Graduate Course offered for the 4th year at NYSBC - Fall 2008

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Cryo electron microscopy in combination with image analysis is increasingly powerful in producing 3D structures of individual molecules and large macromolecular complexes that are unapproachable by other methods.

This comprehensive course will cover the theory and practice of solving molecular structures by electron microscopy. We will start with optics, sample preparation and a basic mathematical description of diffraction before moving into a detailed exploration of the three main methods of structure determination: tomography, single particle analysis, and 2D crystallography. We will end with a discussion of map interpretation and molecular fitting.

This course will have two components

• A series of lectures from local experts followed by student-led discussion sessions.
• A series of practical sessions that parallel the topics introduced during the lectures.

Both components will be held on the premises of the New York Structural Biology Center.

Students and postdocs from all NYC institutions are encouraged to attend
Attendance to the practical sessions will be limited and preference will be given to students enrolled for credit

Textbooks relevant for the course are

• "Three Dimensional Electron Microscopy of Macromolecular Assemblies" by Joachim Frank
• "Electron Crystallography of Biological Macromolecules" by Glaeser, Downing, DeRosier, Chiu

Course Director: David Stokes 212-263-1580 stokes@nyu.edu

Course will involve a 2-hour lecture on Tuesdays 11am-1pm followed by a discussion session relating to the lecture from the prior week from 1-2pm. Practical sessions offering hands on experience with the most important image processing programs will be offered every morning during the week of Nov. 3-7.

Although we don't expect to have any limit to the number of students attending the lectures and discussions, participation in the practical sessions will be limited with preference afforded to students registered for credit.

The New York Structural Biology Center is located on the south campus of City College
89 Convent Ave at 133rd St in Manhattan
A,C,B,D trains to 125th St or 1 train to 137th St

Registration Information

Please notify the course director (David Stokes) of your intention of taking the course.

For graduate students wishing to take the course for credit:

This course will be listed in the catalogue of the New York University School of Medicine (http://www.med.nyu.edu/sackler/courses).

listed as G16.4408 - Cryoelectron Microscopy of of Macromolecular Assemblies.
Call # 31569
3 credits for the lecture/discussion
1 credit for the practical sessions

• NYU students should enroll through the Sackler Inst.
  
• Mt. Sinai students should enroll through NYU GSAS using the above course information.
  
• Albert Einstein College of Med students should contact Hernando Sosa or Todd Evans.
  
• Rockefeller University students should contact Seth Darst or Sid Strickland.
  
• Students from Wadsworth or SUNY Albany should contact Carmen Mannella.
  
• Students from Columbia or CUNY can enroll through the Inter-University Doctoral Consortium
  
• Other students should contact the Dean of Graduate Studies or your institutional representative on the NYSBC CryoEM Operations Committee to learn about procedures for taking this course for credit.
  

Syllabus

Intro to EM Optics, image formation and Diffraction - Sept 16 - David Stokes (NYU/NYSBC)

Fourier transforms, CTF, cross-correlation - Sept 23 - Bill Rice (NYSBC)

Single Particle Analysis I - Sept 30 - Joachim Frank (Columbia University)

Single Particle Analysis II - Oct 7 - Joachim Frank (Columbia University)

Single Particle Analysis III - Oct 14 - Joachim Frank (Columbia University)

Electron Tomography I - Oct 21 - Carmen Mannella (Wadsworth Ctr)

Electron Tomography II - Oct 28 - Carmen Mannella (Wadsworth Ctr)

Practicals Exercises - Nov 3-7

Electron Crystallography I - Nov 11 - Iban Ubarretxena (Mt. Sinai)

Electron Crystallography II - Nov 18 - Iban Ubarretxena (Mt. Sinai)

Helical reconstruction - Nov 25 - Hernando Sosa (Albert Einstein College of Medicine)

Hybrid Methods - Dec 2 - David Stokes (NYU/NYSBC)

Molecular fitting - Dec 9 - Hernando Sosa (Albert Einstein College of Medicine)

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-- DavidStokes - 25 Jul 2008