New York Structural Biology Center
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Progress in Equipment Installations

JEOL 3200FSC Electron Microscope: purchased 6/30/05 -  delivered 1/15/07 -  in service June 2007
    • 300kV FEG gun
    • liquid He, side entry stage
    • in-column "omega" energy filter
    • Gatan 4kx4k Ultrascan CCD camera
    • off-axis TV-rate CCD camera
    • flip-flop rotate tilt specimen cartridge
    • SerialEM tomography software

JEOL 2100F Electron Microscope: purchased 6/30/05 -  delivered 3/8/06 -  in service July 2006
    • 200 kV FEG gun
    • Gatan 626 high-resolution cryo-transfer holder
    • Tietz 2kx2k CCD camera
    • Tietz TV-rate CCD camera
    • Tietz tomography software
    • SerialEM tomography software

JEOL 1230 Electron Microscope: purchased 6/30/05 -  delivered August 15, 2005 -   in service Sept 2005
    • 120kV gun
    • Gatan Erlangshen ES500W CCD camera/li>

Technai G2 F20 cryo Electron Microscope: purchased 9/30/03 -  delivered March 2004 -   in service May 2004
    • 200kV FEG gun
    • Gatan 626 high-resolution cryo-transfer holder
    • Gatan CT3500 high-resolution cryo-transfer holder
    • Gatan CT3500TR tilt-rotate cryo-transfer holder
    • Fischione high field-of-view, single-tilt 80 deg tomography holder
    • Tietz 4kx4k CCD camera
    • Tietz Fastscan TV-rate camera
    • Tietz tomography software
    • SerialEM tomography software

Plunge Freezer: installed Nov 2003
    • liquid ethane used as cryogen
    • vitrification of EM grids <= 10 um thickness
Wohlwend High Pressure Freezer: installed May 2007
Bal-tec High Pressure Freezer: installed Dec 2003
    • liquid nitrogen at 4500 psi used as cryogen
    • vitrification of tissue samples <= 100 um
BOC Edwards AUTO-306 Vacuum Evaporator: installed May 2004
    • oil-free turbo molecular pump
    • carbon evaporation
    • metal evaporation
    • rotary stage
    • glow discharge

Leica Cryoultramicrotome EM UC6/FC6: installed April 2004
    • cryo-sections of frozen tissues (e.g. from high pressure freezer>
    • thin sections of plastic embedded tissues or pellets
Leica Freeze Substitution System EM AFS: installed April 2004
    • three set temperatures between -140oC and +65oC
    • variable ramp times between temperatures
    • ten memory positions for saving protocols
    • UV light for low temperature polymerization
Laser Diffractometer  designed on site and built Jan - March 2004
    • polarizer to adjust intensity
    • CCD camera for viewing transform
    • video recorder for convenient record
Z/I imaging Photoscan film densitometer:  installed Nov 2003
    • high speed
    • test results
PDS 1010GM: installed Jan 2004
    • superior accuracy and optics
    • selectable aperture sizes
    • test results