The JEOL 3200FSC is a state of the art instrument that operates with an accelerating voltage of 300kV. It is equipped with a Schottky field emission gun to provide a highly coherent wave front which illuminates the specimen, which is placed on a small cartridge that is completely immersed in the vacuum of the column, in a chamber that can be cooled either by liquid nitrogen or liquid helium. Further down in the column, an omega energy filter allows the separation of the inelastically scattered electrons to produce zero-loss images, or with the proper fine-tunning, elemental mapping in suitable specimens.

Two cameras are installed on this microscope for high end applications. As in the other field emission instruments at the NYSBC, a Tietz fast scan camera is installed to aid in the search of suitable areas of the specimen under low magnification, low illumination conditions, as well as focusing at high magnifications prior to exposure. A Gatan ultrascan 4kX4k CCD camera can be used for the collection of images or spectra through Digital Micrograph.

The program SerialEM is installed on all the instruments at the NYSBC to provide the capability to collect tilt series for tomographic reconstructions, on a common interface that can be easily learned by the affiliated users.

Specifications for this instrument, as well as operational specifics are available to affiliates of the NYSBC at http://www.nysbc.net/twiki/bin/view/Main/CryoEM

The images on the right hand side correspond to frozen hydrated influenza virus (top), and frozen hydrated stacked disk aggregates of tobacco mosaic virus protein, both energy-filtered.

Instructions for use

Basic Instructions for use

Information and Calibrations