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Principles & Protocols

Protocols - Edwards 306 Vacuum Evaporator

Jump to Principles- Carbon Coating

Jump to Principles- Glow Discharge

Important:

• Please report any bell jar damage. Failure to do so may risk serious injury to others.

General Notes

• Wear gloves when touching sources
• Positive source-ceramic
• Negative source- no ceramic
• It is critical that pos+ is connected to pos+ and neg- to neg- when attaching accessories.

Start Up

• Switch instrument power on (above Rotatilt knob) turn knob from 0 to 1
• Error message will be displayed press reset then start
• Wait for sealed light to appear

Venting the chamber

• Press vent
• Unhinge bell jar
• Carefully lift bell jar
• Add or remove samples and/or accessories

Loading CarbonThread

Loading CarbonRod

MetalShadowing

Pumping down the chamber

• Use compressed air to clean pirani before pumping down
• Carefully close the bell jar
• Lock the safety hinge
• Press cycle to begin pump down

GlowDischarge

Shut down

• Close bell jar
• Press Cycle and wait until high vacuum has been reached
• Press seal
• Press stop
• When the digital display reads STANDBY turn power switch to 0

MaintenanceNotes

Visit Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005