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Cryo-EM home

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Principles & Protocols

Protocols - Microtome Conversion

Remove Cryosectioning unit

• Bake for at least one hour before removing
• Loosen thumb screw on specimen arm
• Loosen both levers on right side
• Unplug cable of left
• Slide cryo unit towards yourself

Attaching room temperature sectioning unit

• Slide plastic sectioning tray until plastic is flush with microtome frame

Removing room temperature unit

• slide plastic tray towards yourself

Attaching cryosectioning unit

• Press "FC Mount" on touch screen
• Raise specimen arm to top position
• Slide Cryo unit onto tracks
• Connect sectioning arm with specimen holder
• Slide cryo unit remainder of the way on
• Leave a gap between back of chamber and heater about the thickness of a penny
• Fasten specimen arm thumb screw
• Make sure hand wheel can make a complete turn
• Fasten two levers on right
• Give a light tug to make sure they are secure
• Fasten cable on left to back of machine
• Connect LN2 hose
• Insert specimen chuck
• Insert knife holder
• Turn on grey control unit
• Cool down

Visit the Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005