Research Overview

Our laboratory uses electron microscopy to study the structural basis for membrane transport and cell-cell adhesion. For membrane transport, we are using electron crystallography to study ordered assemblies of ATP-dependent ion pumps to characterize the organization of domains and conformational changes that accompany the transport cycle. We are also developing methodologies for screening 2D crystallization trials, to make electron crystallography more effective as a general method for structure determination of membrane proteins. For cell adhesion, we are using electron tomography to study the architecture of intercellular junctions and the protein-protein interactions that stabilize these large, cellular protein assemblies in situ.

Lab Members

Principal Investigator
David Stokes

Postdoctoral Associates
Changki Kim
Nicolas Coudray
Paula Upla
Ralph LaSala

Former Members
Alistair Siebert
Martin Vink
Gethin Owen